CITC revised template

1. Basic recipe is as follows. This recipe requires that you make up several solutions ahead of time. These are described below

Ingrediant	amount for 1L	amount for 2.5L	amount for 5L
Proteose Peptone	10 g	25 g	50 g
Trypticase Peptone	5 g	12.5 g	25 g
Ribonucleic Acid	1 g	2.5 g	5 g
MgSO4*7H2O	0.5 g	1.25 g	2.5 g
Phosphatidylethanolamine	10 ml	25 ml	50 ml
Fatty Acids Solution	5 ml	12.5 ml	25 ml
Vitamin Solution	5 ml	12.5 ml	25 ml
Stigmasterol Solution	1 ml	2.5 ml	5 ml
Thioctic Acid	0.5 ml	1.25 ml	2.5 ml

adjust to pH 7.0 with 6M NaOH

1. Stir for 20 min after pHing

2. Autoclave for 20 min.

3. Transfer at intervals of 1 to 2 weeks. After reaching peak density the culture will die off abruptly.

From Nerad, T. A. and Daggett, P-M. 1992. Cultivation of Paramecium. in Protocols in Protozoology edited by J.J. Lee and A. T. Soldo, Published by the Society of Protozoologists

Last updated Monday July 2, 2001 Webmaster Dean Fraga.