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II.
Lab exercises available at this web site.
These
have been modified from hard copies to make them easier to read
as web pages
Lab
exercise 1 Introduction to scientific methodology using Paramecium
This
exercise has students look at whether a single celled organism,
Paramecium, can respond to external stimuli such as food,
light, magnetism, heat and so forth. It is designed to be an introduction
to scientific methodology and is a good exercise to acquaint students
with the use of statistics (mainly the Chi square test).Submitted
by Dr. Dean Fraga (adapted from Dr. Judy Van Houten's work)
Lab
exercise 2 Paramecium Behavior
The
objective of this lab is to introduce students to the need for objective
and quantifiable measurements. The lab simply requires two different
strains of Paramecium grown under the same conditions. It is a good
lab for the first day in Lab because of the concepts involved and
ease of set-up. (specific lab developed by Dr. Dean Fraga, CoW -
dfraga@acs.wooster.edu).Several
behavioral mutants are available
for use in this simple experiment.
Lab
exercise 3 Measuring population growth using Tetrahymena
In
this exercise, students explore the population growth of a unicellular,
asexually reproducing protozoan, Tetrahymena thermophila,
which is chosen because it multiplies very rapidly on a sterile,
easily prepared medium. Students measure the growth of two populations
of this protozoan which were grown under somewhat different conditions.
Submitted by Dr. Joe Frankel (Univ. of Iowa)
Lab
exercise 4 Cell counting
This
exercise introduces student's to manual cell counting techniques.
More importantly, it gives students a feel for important experimental
variables, such as sampling errors, and will require that they
be attentive to seemingly trivial aspects of experimental technique
that often contribute to the reproducibility of a result. Submitted
by Dr. Marty Gorovsky's lab (Univ. of Rochester)
Lab
exercise 5 Basic microscope procedures
The
light microscope (and various modifications of it) is one of the
most fundamental tools of Cell Biology. Learning to use the microscope
correctly will make a number of the later lab periods a lot easier.Submitted
by Dr. Marty Gorovsky's lab (Univ. of Rochester)
Lab
exercise 6 The effect of various drugs upon cilia regeneration in
Tetrahymena
This
is a two part lab designed to acquaint students with organelle
biogeneisis and the use of metabolic inhibitors in cell biology.
On the first day student's conduct an experiments to determine
the sensitivity of Tetrahymena to some drugs that are commonly
used to study the synthesis and assembly of macromolecules in
cells. During this period, students become familiar with the deciliation
process to facilitate an experiment on cilia regeneration during
the next lab period.Submitted by Dr. Marty Gorovsky's lab (Univ.
of Rochester)
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