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Exercise 4 Exercise 4 Overview. You will identify homologs to the Paramecium PP2B sequences in other species. You will then find relevant papers using Entrez. To complete the assignment you must identify twenty amino acid sequences (preferably, full length genes) that are similar to your Paramecium PP2B sequence but come from other species. Try to select PP2B genes from species with completely sequenced genomes (C. elegans, Drosophila, zebrafish, mammals, such as humans). You will construct a table of the sequences identifying each sequence as to Genbank accession number, protein phosphatase isoform (if known) and species. For each, indicate whether there is an original paper that describes the sequenes (as to function or as to how it was originally identified). Give the complete references for each paper (with titles) as a bibliography, not in the table. These can often be found in the Genbank file. The point of this analysis is to gather information to gain clues as to the function of the Paramecium genes. Once you have some ideas about function, you can begin to see how these 'translate' to Paramecium. Ultimately, for the grant exercise you will devising an experimental approach using RNAi that will identify the function(s) of the full length PP2B genes identified in Paramecium. For this assignment (Exercise 4) you will be essentialy generating material you can use in Exercise 5.
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