| Week |
Goal |
What's
due when |
|
Week 1
|
Lab
discussion: Introduction to the Grant writing project.
Introduction
to Paramecium functional
genomics project.
Test
of library skills. |
Assignment 1
- Due on Friday
|
|
Week 2
|
Lab discussion:
How to do alignments using MacVector.
Identifying introns in PP2b genes (class project). We will
create a poster summarizing our data. Grant discussion: Q&A
|
Assignment 2 – Due
day of class.
Assignment 3 -
Due on Friday.
|
|
Week 3
|
Lab exercise: Continue poster creation.
|
Assignment
4 – due
on Friday
|
|
Week 4
|
Discussion of RNAi paper in class
Final primer designs due – order on Friday
|
Identify Assignment
6 partners Schedule meetings with Dr. Fraga Schedule
as working pairs.
Assignment 4 returned |
|
Week 5
|
Information Literacy session |
Assignment
5. Due on following Monday by 5:00 PM |
|
Week 6 (schedule
individual meetings)
|
Working
with Paramecium – basic culturing
techniques
Isolation of RNA from Paramecium cells in defined amounts.
What
should be in your notebook? |
Return Assignment 5 on Friday
|
|
Week 7
|
Lab
exercise: PCR using primers
|
Nothing is due
from lab (see grant syllabus for grant due dates)
|
|
Week 8
|
Lab exercise: Run gel of PCR samples |
Make sure you
have an image of the gel for inclusion in your lab notebook
and grant
|
|
Week 9
|
RNAi introduction
|
Lab report of your results from the lab exercise is due
on Friday
|
|
Week 10
|
Organize pilot RNAi experiments (set up by Dr. Fraga)
|
Yoru lab notebook
should have clear discussion of the RNAi experiments and
a detailed timetable. |
|
Week 11
|
Conduct RNAi experiment
|
You should
have your data collected in your notebook and organized
clearly |
|
Week 12
|
Buffer:
Conduct additional RNAi experiment
Optional – isolate RNA; measure RNAi effect
|
Make sure you have some data to include in your grant as
preliminary results section so that you can get a critique
of how you are presenting it. |
|
Week 13
|
Data analysis/real-time
PCR?
|
Try
to write up your results from the experiment as a preliminary
results section. |
|
Week 14
|
Buffer |
Continue refining your preliminary results section |
| Week 15 |
Finals week
|
Grant
due on day of finals at 5:00 PM |
| |
|
|
Link
to Grant Tips
Assignments 1-5
should be done individually. During week four we will
identify who will be partners for the grant project. However,
I want assignment
4 and 5 to be written individually so that I can assess the
writing skills of each partner. The grant (assignment 6) can
be done
in
pairs but throughout the assignment I will ask who wrote what
and what each individual's contribution was. You will be required
to evaluate your partner at the end of the assignment.
I will require that
all drafts be collected in one notebook to be handed in at the
end of the year. You should include all reviewer's comments and
any responses you may have had to those comments. In addition,
I request that you keep all of your collected materials in a separate
notebook so that I can review it.
Copies
of the previous year's grants are available for copying upon request.
Total points from
the exercise - 200 points (Group project, Cumulative Grade)
