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What should be in my lab notebook?

The lab notebook will be checked at random intervals.

I am asking that you keep a dated and detailed notebook for the laboratory portion of this class. It should be a standard notebook such as can be obtained at the bookstore. It should not a three ring binder nor have loose paper stuck in it. The lab notebook should provide detailed information about the experiments you conducted in class. The types of things I will be looking for are:

• consistent entries
• dated entries
• complete entries
• raw and summarized data where appropriate
• protocols that can be read and followed

In addition, I will be looking for specific elements that reflect the experiments we will be cinducting in class. The specific elements I will be looking for in the notebook when I evaluate it will be

• First experiment: Should have a detailed summary of how RNA was isolated and the PCR reaction conducted.
• Second experiment: Detailed proptocol for RNAI and your assay. Diary of RNAi experiments with results (raw data and experimental design)
• Optional experiment: Protocol for RNA isolation and results (raw data, basically yield of RNA isolated). This will be for extra credit. Should include the protocol for real-time PCR and results (raw data)