Genetic Analysis in C. elegans
Assignments
Week 1 | Week 2 |
Week 3 | Week 4
Week 1: Introduction to C. elegans
Tuesday:
- Before lab, read the following sections of the Lab Manual:
Thursday:
- Before lab, read the following sections of the Lab Manual:
- In the lab, perform
Lab Instructions #1.
Week 2: Screen for suppressor mutations
Tuesday:
- Before lab...
- Read Brenner (1974) "The genetics of Caenorhabditis
elegans". Genetics77: 71 - 94.
- In the Study Guide for
Brenner (1974), answer the questions in Part I.
Answers are due at the start of the
lab session.
- In the lab...
- Examine the cross set up for
Lab Instructions
#1.
- Begin a screen (or search) for suppressor mutations of the
uncoordinated mutation unc-93. The mutant animals
exhibit very little movement, making it relatively easy to
identify suppressors that restore wild-type locomotion.
Thursday:
- Before lab...
- In the lab...
- Begin the crosses to determine the nature and linkage of
the suppressor mutation.
Week 3: Genetic Analysis
Tuesday:
- In the lab...
- Continue your crosses to determine the nature and linkage
of the suppressor mutation.
Thursday:
- Before lab...
- In the lab...
- Continue your crosses to determine the nature and linkage
of the suppressor mutation.
Week 4: Genetic Analysis
(continued)
- In the lab...
- Complete your crosses to determine the nature and linkage
of the suppressor mutation.
- Develop a strategy to determine if your unknown mutation is
allelic to a suppressor gene identified by Greenwald and
Horvitz (1980).
One week later, an individually prepared research proposal
is due. This proposal must be prepared as described in
Pechenik (1993).
Two weeks later, a revised lab report, prepared jointly if
desired, is due.
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Analysis in C. elegans
Genetic Analysis in C. elegans
Last Updated: October 2001
William R. Morgan
wmorgan@acs.wooster.edu