Charles L. Borders, Jr.

Charles L. Borders, Jr. is a Professor of Chemistry Emeritus. Montie, as he is known to his friends, received his B.A. from Bellarmine College in 1964 and his Ph.D. from Caltech in 1968. His research interests include the identification of essential amino acids at enzyme active sites using site-directed mutagenesis, chemical modification, and enzyme kinetics. He is also interested in the use of molecular graphics to explore protein structure. He has taken one-year research leaves at Harvard Medical School, the Carlsberg Laboratory in Copenhagen, Duke Medical School, Pennsylvania State University, the University of California at San Francisco and the University of North Carolina at Chapel Hill. His teaching responsibilities included biochemistry and introductory organic chemistry. He enjoys gardening, chopping wood, and listening to bluegrass music, a legacy of his Kentucky upbringing.

Recent publications:

T. D. Wood, Z. Guan, C. L. Borders, Jr., L. H. Chen, G. L. Kenyon and F. W. McLafferty; Creatine Kinase: Essential Arginine Residues at the Nucleotide Binding Site Identified by Chemical Modification and High-Resolution Mass Spectrometry; Proc. Natl. Acad. Sci. U.S.A., 1998, 95, 3362-3365.

C. L. Borders, Jr., M. J. Bjerrum, M. A. Schirmer and S. G. Oliver;; Characterization of Recombinant Saccharomyces cerevisiae Manganese-Containing Superoxide Dismutase and Its H30A and K170R Mutants Expressed in Escherichia coli; Biochemistry, 1998, 37, 11323-11331.

L. H. Chen, C. L. Borders, Jr., J. R. Vasquez, and G. L. Kenyon; Rabbit Muscle Creatine Kinase: Consequences of the Mutagenesis of Conserved Histidine Residues; Biochemistry, 1995, 35, 7895-7902.

C. L. Borders, Jr., J. A. Broadwater*, P. A. Bekeny*, J. E. Salmon*, A. S. Lee*, A. M. Eldridge* and V. B. Pett; A Structural Role for Arginine in Proteins: Multiple Hydrogen Bonds to Backbone Carbonyl Oxygens; Protein Sci., 1994, 250, 541-548.

* Wooster undergraduate.

Invited Lectures and Papers/Posters Presented at Professional Meetings:

C. L. Borders, Jr.; A Multifaceted Approach to the Study of Transition State Stabilization in Kinases, Department of Chemistry, University of North Carolina at Chapel Hill, March 2000.

C. L. Borders, Jr.; Structure/Function Relationships of Conserved Residues in Creatine Kinase by Site-Directed Mutagenesis, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, March 2000.

C. L. Borders, Jr.; Transition State Stabilization at the Active Site of Kinases; Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill, NC, March 1999.

C. L. Borders, Jr.; Transition State Stabilization at the Active Site of Kinases; Department of Physiology and Biophysics, Wright State University School of Medicine, Dayton, OH, May 1999.

P. L. Edmiston, K. L. Schavolt*, E. R. K. Gbeddy*, M. J. Thomenius*, E. A. Kersteen*, N. R. Moore* and C. L. Borders, Jr.; Kinetic Analysis of Arginine-285 Mutants of Rabbit Muscle Creatine Kinase; 16th Annual Enzymes Mechanisms Conference, Napa, CA, January 1999.

V. B. Pett, B.-L. Zhou*, H. A. Rowell*, and C. L. Borders, Jr.; New Examples of Structural Arginine in Proteins; International Conference on Protein Folding and Design, National Institutes of Health, Bethesda, MD, April 1996.

C. L. Borders, Jr.; Creatine Kinase: The Roles of Conserved Arginines by Site-Directed Mutagenesis and Enzyme Kinetic Analysis; Department of Chemistry, University of Akron, Akron, OH, March 1996.

C. L. Borders, Jr.; Creatine Kinase: The Roles of Conserved Arginines by Site-Directed Mutagenesis and Enzyme Kinetic Analysis; Department of Chemistry, Denison University, Granville, OH, February 1996.

L. H. Chen, C. L. Borders, Jr., J. R. Vasquez and G. L. Kenyon; Mutagenesis of Conserved Histidines in Rabbit Muscle Creatine Kinase; Joint Meeting of the American Society of Biochemistry and Molecular Biology and the Division of Biological Chemistry at the American Chemical Society, San Francisco, California, May 1995.

* Wooster undergraduate.

Recent Grant Support:

"Creatine Kinase: Structure/Function Relationships of Conserved Residues by Site-Directed Mutagenesis," NSF-RUI, $192,000, by C. L. Borders, Jr. and P. L. Edmiston

Research Corporation, 1996-1998, $34,500, Creatine Kinase: The Role of Conserved Arginines by Site-Directed Mutagenesis.

Camille and Henry Dreyfus Foundation Scholar/Fellow Program for Undergraduate Institutions, 1997-1999, $60,000.